Rabbit models of recurrent aphthous ulcer replenished with superoxide dismutase

doi:10.3969/j.issn.2095-4344.2014.49.025

Abstract

Abstract:

BACKGROUND: The precise pathogenesis of recurrent aphthous ulcers remains controversial in present medical science. Oxygen free radical increase in the body is one of the causes of recurrent aphthous ulcers.

OBJECTIVE: To observe the effects of superoxide dismutase on the therapeutic effect of recurrent aphthous ulcers.

METHODS: After establishing 24 rabbit models of recurrent aphthous ulcers, they were randomly assigned to three groups. Model control group as a contrast was not treated. Rabbits in the negative control group were cleaned with iodoglycerin in local region daily. Rabbits in the superoxide dismutase group were intragastrically administered with superoxide dismutase capsule solution. Local ulcer was cleaned with iodoglycerin. One week later, tissue with oral ulcer lesions was obtained and compared. In each group, after model establishment, blood was collected from rabbit ear vein at the same time in the morning every 4 days. Flow cytometry was used to analyze the changes in T cell subsets CD3⁺, CD4⁺, CD8⁺, and CD3⁺/CD8⁺.

RESULTS AND CONCLUSION: When the ulcer lesions formed, peripheral blood CD3⁺, CD4⁺, CD4⁺/CD8⁺ ratio through statistics was apparently lower than normal levels in the model control group and negative control group. However, the number of CD8⁺ cells was greatly more than normal value. In the superoxide dismutase group, after administrated with superoxide dismutase capsule, the number of CD3⁺ and CD4⁺ cells was gradually increased, but the number of CD8⁺ cells showed a decreased trend. These data suggested that natural scavenger superoxide dismutase using oxygen free radical can effectively scavenge excessive oxygen free radical in the ulcers, resulting in treatment of recurrent aphthous ulcers.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: oral ulcer, superoxide dismutase, models, animal

CLC Number:

R318

Hu Jing, Zhu Jian-hua, Wang Shuai. Rabbit models of recurrent aphthous ulcer replenished with superoxide dismutase [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(49): 8015-8019.

Figures/Tables 2

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